《植物生理学报》 2011, 47(5): 512-520

不同光质对烟草叶片生长及光合作用的影响

柯学^1,2,*, 李军营^3,*, 李向阳³, 邬春芳⁴, 徐超华², 晋艳³, 龚明^2,**

1中国农业大学水利与土木工程学院, 北京100083; 2云南师范大学生命科学学院, 生物能源持续开发利用教育部工程研究中 心, 云南省生物质能与环境生物技术重点实验室, 昆明650092; 3云南省烟草农业科学研究院, 云南玉溪653100; 4中国农业科 学院农业资源与农业区划研究所, 北京100081

通信作者：柯；E-mail: gongming63@163.com；Tel: 0871-5516516

摘　要：

通过对烟草植株覆盖白、红、黄、蓝、紫色滤膜获得不同光质, 研究了光质对烟叶生长及光合作用的影响。结果表 明, 与白膜处理相比, 红膜与蓝膜处理下的烟草叶片较厚, 比叶面积较小, 叶绿素a/b比值、净光合速率、可变荧光强度(Fv) 和最大荧光强度(Fm)的比值Fv/Fm (PSII最大光化学量子效率)、PSII实际光化学量子效率(ΦPSII)、光饱和点和CO2饱和点均 较高。黄膜处理下的叶片较白膜处理的更薄, 净光合速率、Fv/Fm、ΦPSII、光饱和点、CO2饱和点均较低。紫膜处理的叶片 比叶面积比白膜处理的小, 净光合速率和Fv/Fm比白膜的大。实验结果表明红光、蓝光和紫光促进了烟叶的生长, 这种促进 作用是与其高光合效率紧密相连的; 而黄光对烟叶的生长有一定程度的抑制作用。

关键词：光质; 烟草叶片; 生长; 光合作用; 叶绿素荧光

收稿：2011-03-01   修定：2011-03-30

资助：国家自然科学基金(30460016)和中国烟草总公司云南省公 司科技计划项目(2010YN03和07A01)。

Effects of Different Light Quality on Growth and Photosynthesis of Tobacco

KE Xue^1,2,*, LI Jun-Ying^3,*,LI Xiang-Yang³, WU Chun-Fang⁴, XU Chao-Hua², JIN Yan³, GONG Ming^2,**

¹College of Water Conservancy & Civil Engineering, China Agricultural University, Beijing 100083, China; ²Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, School of Life Sciences, Yunnan Normal University, Kunming 650092, China; ³Yunnan Academy of Tobacco Agricultural Sciences, Yuxi, Yunnan 653100, China; ⁴Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Corresponding author: KE Xue; E-mail: gongming63@163.com; Tel: 0871-5516516

Abstract:

By covering tobacco plants with white, red, yellow, blue and purple films to obtain different light quality, the effects of light quality on growth and photosynthesis of tobacco leaves were investigated. The results showed that, compared with white film treatment, red and blue film treatments increased leaf thickness, lowered specific leaf area (SLA), raised the ratio of chlorophyll a/b (Chl a/b), net photosynthetic rate (Pn), the ratio (Fv/Fm) of variable fluorescence intensity (Fv) and maximal fluorescence intensity (Fm), the photosynthetic quantum yield of photosystem II (Φ_PSII), and light and CO₂ saturation points in tobacco leaves. On the contrary, yellow film treatment led to thinner leaves and lower Pn, Fv/Fm, Φ_PSII, and light and CO₂ saturation points in the tobacco leaves. In addition, purple film treatment demonstrated less SLA and higher Pn and than white film did. In general, red, blue and purple light promoted the growth of tobacco leaves, which was related to higher photosynthetic efficiency; and yellow light inhibited the growth of tobacco leaves to some extent.

Key words: light quality; tobacco leaves; growth; photosynthesis; chlorophyll fluorescence